LOCUS       62056_11700        5852 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             369..2018
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             2025..2072
                     /codon_start=1
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             2076..2195
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(2244..2365)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2559..2916
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2947..3543
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3571..3619
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3946..4534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4737..5594)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5761..5852
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagtg ccaggtgaat ggaagtcccg
       61 caggccggaa gtggacgagc ctactcgccc gggcgcgggg gggcgcaaga gcgcgcagca
      121 tcttcattga ggaacccggg cggcgaacat ggagttccat gtgcgtctta tgtaaagaga
      181 gcgacgggcg tctccaccaa ttgatgagcg tagctctcag gttccgcccc gccagtatgc
      241 aaatgaggtg gcactccgac caatggcgcg cgcaggggcg ggctcagcgc gcgcggtcac
      301 gttttccact atgtgacagc ggagggcgac gcgaagcttg gcaatccggt actgttggta
      361 aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg
      421 aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg
      481 gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg
      541 agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga
      601 tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca
      661 tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca
      721 tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca
      781 acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact
      841 accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg
      901 agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca
      961 gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc
     1021 gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc
     1081 tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct
     1141 gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc
     1201 aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga
     1261 gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc
     1321 cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc
     1381 agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca
     1441 agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca
     1501 ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca
     1561 tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc
     1621 tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc
     1681 tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc
     1741 tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg
     1801 gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga
     1861 tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt
     1921 tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga
     1981 ttctcattaa ggccaagaag ggcggcaaga tcgccgtgaa ttctgcttgc aagaactggt
     2041 tcagtagctt aagccacttt gtgatccacc ttaacagcca cggcttccct cccgaggtgg
     2101 aggagcaggc cgccggcacc ctgcccatga gctgcgccca ggagagcggc atggatagac
     2161 accctgctgc ttgcgccagc gccaggatca acgtctaact gcagtctaga gtcggggcgg
     2221 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2281 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2341 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2401 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2461 cgataaggat ccgtttgcgt attgggcgct cttccgctga tctgcgcagc accatggcct
     2521 gaaataacct ctgaaagagg aacttggtta gctaccttct gaggcggaaa gaaccagctg
     2581 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2641 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2701 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2761 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2821 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     2881 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcgattcttc tgacactagc
     2941 gccaccatga ccgagtacaa gcctaccgtg cgcctggcca ctcgcgatga tgtgccccgc
     3001 gccgtccgca ctctggccgc cgctttcgcc gactaccccg ctacccggca caccgtggac
     3061 cccgaccggc acatcgagcg tgtgacagag ttgcaggagc tgttcctgac ccgcgtcggg
     3121 ctggacatcg gcaaggtgtg ggtagccgac gacggcgcgg ccgtggccgt gtggactacc
     3181 cccgagagcg ttgaggccgg cgccgtgttc gccgagatcg gcccccgaat ggccgagctg
     3241 agcggcagcc gcctggccgc ccagcagcaa atggagggcc tgcttgcccc ccatcgtccc
     3301 aaggagcctg cctggtttct ggccactgta ggagtgagcc ccgaccacca gggcaagggc
     3361 ttgggcagcg ccgtcgtgtt gcccggcgta gaggccgccg aacgcgccgg tgtgcccgcc
     3421 tttctcgaaa caagcgcacc aagaaacctt ccattctacg agcgcctggg cttcaccgtg
     3481 accgccgatg tcgaggtgcc cgagggacct aggacctggt gtatgacacg aaaacctggc
     3541 gcctaatgat ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
     3601 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
     3661 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3721 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4381 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4681 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     4741 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     4801 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     4861 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     4921 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     4981 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     5041 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     5101 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     5161 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     5221 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     5281 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     5341 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     5401 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     5461 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     5521 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     5581 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     5641 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     5701 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     5761 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     5821 cagtgcaagt gcaggtgcca gaacatttct ct
//
